Journal: The Journal of Experimental Medicine

Article Title: GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions

doi: 10.1084/jem.20090866

Figure Lengend Snippet: PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with BrdU at 2 h before treatment with PTx or PBS (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.

Article Snippet: In BrdU labeling experiments, mice received a single 0.2-ml i.v. injection of 2 mg BrdU in PBS (BD).

Techniques: Flow Cytometry, Injection, Labeling, Derivative Assay

Journal: PLoS ONE

Article Title: Potential Relevance of α 1 -Adrenergic Receptor Autoantibodies in Refractory Hypertension

doi: 10.1371/journal.pone.0003742

Figure Lengend Snippet: (A) α 1 -AAB isolated from the serum of a patient with refractory hypertension elicited a Ca 2+ signal. (B) The rabbit α 1 -AB gave a similar Ca 2+ signal. (C) A human control IgG preparation was unable to affect intracellular Ca 2+ . Cardiomyocytes were electrically stimulated at 1 Hz, and the peak Ca 2+ was monitored.

Article Snippet: Following, vessel rings were incubated with α 1 -AAB (5 µg in 5 ml PBS buffer), rabbit α 1 -AB (50 µg in 5 ml PBS buffer), rabbit IgG (50 µg in 5 ml PBS buffer, Dunn Labortechnik GmBH, Asbach, Germany) and human control IgG endobulin (50 µg in 5 ml PBS buffer).

Techniques: Isolation

Journal: PLoS ONE

Article Title: Potential Relevance of α 1 -Adrenergic Receptor Autoantibodies in Refractory Hypertension

doi: 10.1371/journal.pone.0003742

Figure Lengend Snippet: A brisk KCl response is documented. (A) Representative experiment showing the contractile response to phenylephrine (PE, 10 nM–10 µM) and rabbit α 1 -AB (50 µg in 5 ml PBS buffer). The PE and antibody responses were similar in kind. (B) Contractile response of human α 1 -AAB isolated from two patients (5 µg in 5 ml PBS buffer) and rabbit α 1 -AB (50 µg in 5 ml PBS buffer) are compared to the PE response (300 nM and 10 µM, respectively) is shown. Contractions are expressed as % KCl response from patient 1 and patient 2. The human antibody responses were less than the low and high-dose PE responses. The rabbit α 1 -AB responses approached the PE responses. Two columns on the right are control human and control rabbit IgG showing no response.

Article Snippet: Following, vessel rings were incubated with α 1 -AAB (5 µg in 5 ml PBS buffer), rabbit α 1 -AB (50 µg in 5 ml PBS buffer), rabbit IgG (50 µg in 5 ml PBS buffer, Dunn Labortechnik GmBH, Asbach, Germany) and human control IgG endobulin (50 µg in 5 ml PBS buffer).

Techniques: Isolation